3D reconstruction of axonal projection map from an auditory belt area

• A viral tracer, which works as an anterograde tracer by expressing fluorescent proteins in infected neurons, was injected to an auditory belt area of an adult marmoset
• The injection site was histologically confirmed to be in auditory belt area CL, according to myelin and Nissl substance staining

• During sectioning, every time before each brain section was sliced, a block-face image was stored as the original form of the brain section
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• Fluorescent images were acquired by a slide scanner (NanoZoomer 2.0 HT, Hamamatsu)
• The fluorescent image of each brain section was transformed to the corresponding block-face image using a program developed for MRI studies (ANTs; Avants et al., 2011)
• A stack of the transformed images becomes a 3D reconstruction of the fluorescent images and was rendered using Fluorender (Wang, Otsuna et al., 2009)

[DataID: 2096. Creator Hiroshi Abe]