Brain/MINDS is making a connection map of the marmoset cortex using anterograde tracer injection with virus vectors. In the first phase of the project (2014-2018), three groups, led by Tetsuo Yamamori, Noritaka Ichinohe, and Partha Mitra, were involved. The Yamamori team used serial two-photon tomography (STPT) for imaging the projections while Ichinohe and Mitra groups both used a digital slide scanner. For accurate 3D registration, all of the tracer-injected brains were imaged with an MRI-scanner before sectioning. The tracer signals were mapped to a reference 3D space, which will be used for integration of cross-modal experiments, including MRI, DTI and histological staining, in addition to the tracer data. In the second phase of the project (2019-2023) the tracer injection work has been consolidated and is continued on by Yamamori Team.

Yamamori Team, RIKEN CBS

Ichinohe Team, National Institute of Neuroscience

Mitra Team, Cold Spring Harbor Laboratory